rabbit monoclonal primary antibodies against p21  (Proteintech)

Journal: Oncology reports

Article Title: MicroRNA-134-3p inhibits ovarian cancer progression by targeting flap structure-specific endonuclease 1 in vitro.

doi: 10.3892/or.2020.7844

Figure Lengend Snippet: Figure 2. miR-134-3p facilitates cell apoptosis and induces cell cycle arrest at the G0/G1 phase in SKOV-3 and OVCAR-3 cells. SKOV-3 and OVCAR-3 cells were transfected with miR-134-3p mimic or NC mimic. (A) TUNEL assay was performed to assess cell apoptosis (scale bar, 200 µm). (B) Western blot analysis was used to assess the protein expression levels of Blc-2, Bax, cleaved caspase-3 and cleaved caspase-9. (C) Flow cytometry assay was performed to assess the cell cycle. (D) Western blot analysis was used to assess the protein expression levels of cyclin D1, CDK2 and p21. The data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. NC mimic. PCNA, proliferating cell nuclear antigen; miR, microRNA; NC, negative control.

Article Snippet: The membranes were then blocked with TBS-Tween (TBST; 0.1% Tween-20) containing 1% skim milk powder at room temperature for 1 h, and then incubated with rabbit monoclonal primary antibodies against human p21 (1:1,000; cat. no. 2947S), cyclooxygenase-2 (Cox-2; 1:1,000; cat. no. 12282T), matrix metalloproteinase (MMP)2 (1:1,000; cat. no. 40994S), MMP9 (1:1,000; cat. no. 13667S), cyclin d1 (1:1,000; cat. no. 55506S), CdK2 (1:1,000; cat. no. 2546S), Bax (1:1,000; cat. no. 5023S), cleaved caspase-3 (1:1,000; cat. no. 9654S), cleaved caspase-9 (1:1,000; cat. no. 20750S), Bcl-2 (1:1,000; cat. no. 4223S), β-actin (1:1,000; cat. no. 4970T) and FEN1 (1:2,000; cat. no. 82354S) (all from Cell Signaling Technology, Inc.) at 4 ̊C overnight.

Techniques: Transfection, TUNEL Assay, Western Blot, Expressing, Flow Cytometry, Negative Control